Plant Complex Map Files

• Protein Complexes (Excel format, with annotations and protein elution profiles)
  • Description: Annotated complexes generated from walktrap diffusion clustering of FDR < 10% interactions (CF-MS score cutoff 0.509)
• Protein Complexes (Text format, no annotations or elution profiles)
  • Description: Plain text complexes generated from walktrap diffusion clustering of FDR < 10% interactions (CF-MS score cutoff 0.509)
  • Note: The leftmost columns are more inclusive (larger) clusters, rightmost are more restrictive (smaller)
  • More restrictive clustering tends to separate complexes into subcomplexes
  • Cluster numbers give guidance as to which proteins are likely to belong to the same complex. Note that the numbers are only informative within the same column.
• All scored pairwise protein-protein interactions with CF-MS scores (3,076,999 unique pairwise interactions)
  • Description: Scores between Orthogroups with the corresponding CF-MS score and eggNOG generated orthogroup descriptions.
  • Note: Only the highest scoring pairs are considered significant. A CF-MS score >= 0.509 corresponds to 10% FDR, >= 0.207 corresponds to 50% FDR
  • Format: OrthogroupID1 [tab] OrthogroupID2 [tab] Score [tab] Annotation1 [tab] Annotation2

Additional database input files

• Orthogroup Annotations
  • Description: Annotations for all orthogroups
• Translation Table (i.e., the precise orthogroup definitions used for this analysis)
  • Mapping of eggNOG virNOG OrthogroupID to ProteinIDs used in bioinformatics pipeline
  • Generated with EggNOG mapper against EggNOG4.0 orthogroups
  • Format: OrthogroupID,ProteinID,Species code
• Translation Table (contains additional mappings used for web site queries)
  • Mapping of eggNOG virNOG OrthogroupID to different encodings of ProteinID
  • TAIR AT locus, uniprot Accession, uniprot Entry name, Rice locus, Arabidopsis gene name
  • Format: OrthogroupID,ProteinID,Species code,Identifier type

Metadata

• Experiment metadata
  • Description of each fractionation experiment
  • Format: experiment_name,ExperimentID_order,tissue,experiment_type,spec,ExperimentID
• Fraction metadata
  • Description of each fraction in all fractionation experiment
  • FractionID,frac_order,ExperimentID

Test and training interactions

Identifiers (OrthogroupIDs) are eggNOG viridiplantae orthogroup identifiers

• Train Positive PPIs
  • Description: List of training positive ppis used in protein complex discovery pipeline
  • Contain PPI from CORUM human protein complexes transfered to plant ortholog groups, as well as other supported PPI from TAIR
  • Format: OrthogroupID [tab] OrthogroupID
• Train Negative PPIs
  • Description: List of train negative ppis used in protein complex discovery pipeline
  • Note: Since no truly non-interacting proteins exist, these are randomly sampled from observed proteins in our data set
  • Format: OrthogroupID [tab] OrthogroupID
• Test Positive PPIs
  • Description: List of test positive ppis used in protein complex discovery pipeline
  • Format: OrthogroupID [tab] OrthogroupID
• Test Negative PPIs
  • Description: List of test negative ppis used in protein complex discovery pipeline
  • Note: Since no truly non-interacting proteins exist, these are randomly sampled from observed proteinsin our data set
  • Format: OrthogroupID [tab] OrthogroupID

Elution table

• Concatenated elution profiles (125 MB)
  • Description: Table of concatenated elution profiles reported per-fractionation-normalized peptide spectral matches (PSMs) for all observed orthogroups
  • Format: OrthogroupID,[Fractions]
• Tidy elution profile (455MB)
  • Description: Tidy format concatenated elution profiles of all orthogroups
  • Includes a variety of abundance measurements and normalizations
  • fracnorm = normalized by each fraction
  • expnorm = normalized by fractionation experiment
  • Format: filename,ExperimentID,FractionID,ID,Total_PeptideCount,abundance_ppm,spec,fracnorm_PeptideCount,expnorm_PeptideCount,expnorm_ppm,fracexpnorm_PeptideCount
• Link to additional elution data files in Zenodo repository
  • Orthogroup and protein elutions for each experiment and species
  • Raw, per-fractionation-normalized, and parts-per-million peptide spectral matches (PSMs) peptide counts
  • Wide and tidy formatted elutions

Feature Matrix

• Feature Matrix (gz) (3GB)
  • Description: All calculated features with training labels from McWhite & Papoulas et al.
  • Format: OrthogroupID1[space]OrthogroupID2,[features],OrthogroupID1,OrthogroupID2,label

• Feature Matrix (gz) (1.4GB)
  • Description: Feature-selected top 100 features with training labels from McWhite & Papoulas et al.
  • Format: OrthogroupID1[space]OrthogroupID2,[features],label
  • Note: Orthogroup1,Orthogroup2 are in alphatical order

Code

• Plant Protein Complex Map Zenodo repository
• Notes: Zenodo repository contains all files, instructions to replicate, and internal scripts
• Project makes use of following github repositories
• Peptide detection by orthogroup (GitHub)
• Scripts for calculating interaction networks

License

• CC BY 4.0
• Data associated with this website are free to download and share. They are governed by the Creative Commons Attribution 4.0 International (CC BY 4.0) license, which means that they are a part of the public domain, and every use of them is allowed, provided that you give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. If you make extensive use of data from this data set, please credit the authors (see about for references).